This course covers the basic principles of cell structure and function, mainly in and eukaryotic cells. Subjects include the cytoskeleton, the plasma membrane and extracellular matrix, transport, the nucleus, ER, Golgi apparatus, cell communication, stem cells, cell death, cancer and organization of tissues. The functioning of particular cell types will be addressed. The course will also emphasize methods for imaging cells and their components and the computational methods to perform data analysis of images. Integration of the physiological information as well as to reinforce the interest of the students in the use of bioinformatics applications as a necessary tool to approach complex biological and physiological process. The course will also introduce students to the basic principles of light microscopy and the quantitative analysis of fluorescence microscopy images and their application in the study of the cell¿s biology.
Teachers
Person in charge
Berta Alsina Español (
)
Others
Oriol Gallego Moli (
)
Weekly hours
Theory
1.8
Problems
1.4
Laboratory
0.8
Guided learning
0
Autonomous learning
6
Learning Outcomes
Learning Outcomes
Knowledge
K1 - Recognize the basic principles of biology, from cellular to organism scale, and how these are related to current knowledge in the fields of bioinformatics, data analysis, and machine learning; thus achieving an interdisciplinary vision with special emphasis on biomedical applications.
K6 - Recognize the ethical problems that arise from advances in the knowledge and in the application of biological concepts and their computational processing.
Skills
S6 - Identify and interpret relevant data, within the area of study, to make judgments that include social, scientific or ethical reflections.
S8 - Make decisions, and defend them with arguments, in the resolution of problems in the areas of biology, as well as, within the appropriate fields, health sciences, computer sciences and experimental sciences.
S9 - Exploit biological and biomedical information to transform it into knowledge; in particular, extract and analyze information from databases to solve new biological and biomedical problems.
Competences
C6 - Detect deficiencies in the own knowledge and overcome them through critical reflection and the choice of the best action to expand this knowledge.
C7 - Detect, from within the scope of the degree, inequalities based on sex and gender in society; integrate the different needs and preferences based on sex and gender in the design of solutions and problem solving.
Objectives
Introduce the student into the world of Cellular Biology and Physiological phenomenon, to understand the inside world of cells and their organization of higher order systems.
Related competences:
C6,
K1,
S6,
S8,
S9,
Teach to student the bases of basic research in cellular biology and help them reaching the correct conclusions from their experimental results.
Related competences:
C6,
C7,
K1,
S6,
S8,
S9,
To provide students the basic theoretical basic fundamentals, competences and practical skills that allow them to approach light microscopy imaging.
Related competences:
K1,
K6,
S6,
S8,
S9,
Contents
BASICS OF EUKARYOTE CELL: FROM STRUCTURE TO ORGANIZATION
Plasma membrane, transport and ECM. Cell adhesion, the cytoskeleton and the nucleus. The Endoplasmic Reticulum and Golgi. The nucleus and gene regulation. Cellular Communication between animal cells and particular pathways. Pluripotency, Stem Cells and Cancer. Cell death and senescence.
Principles of light microscopy and image analysis in Cell biology
Principles Microscopy I and II. Imaging subcellular structures I and II. Limit of Resolution.
The methodology combines Theoretical lessons, Seminars (with 20-30 students in each group) and Laboratory Practicals (the class is divided in 3 symmetric groups). We will follow a continuous assessment through the delivery of group projects, oral presentations and other evaluations activities. Ultimately, the students knowledge and skills will be evaluated with a final exam.
Evaluation methodology
A series of exams are used to measure the success in meeting the course learning objectives. In order to successfully complete this course, the student must pass at least with 5 points over 10. All exams are compulsory.
The final grade of the students will result from the sum of Cell Biology (up to 6 points) and Microscopy and Image Analysis (up to 4 points). Each of the grades will result from:
Cell Biology:
Essay fo cell biology (20%)
Exam of Laboratory practices- (10%)
Oral Presentations (20%)
Final Exam (Pem and short questions) (50%)
Microscopy and Image Analysis:
Macro for image analysis (70%). This is the result of the sum of Group Macro (up to 3 points), Oral exam (up to 4 points)
and Autoevaluation (up to 3 points)
Integration of Concepts (10%)
Evaluation on Principles of microscopy (20%)
The average grade of the Final exam and the Evaluation on Principles of microscopy must be at least of 3.5 (that is, (0.5*6*EF+0.2*4*EP)/(0.5*6+0.2*4) must be at least 3.5) to consider the rest of the assessment items in the final grade.
Bibliography
Basic:
Molecular Biology of the Cell -
ALBERTS, B.; JOHNSON, A.; LEWIS, J.; RAFF, M.; ROBERTS, K.; WALTER, P. ,
Garland Science, , 2008.
The Cell. A molecular approach -
COOPER, G.M. i HAUSMAN, R.E. ,
Washington D.C. and Sunderland, 2007.
Histología. Texto y Atlas. -
GARTNER, L. P.; HIATT, J. L. ,
McGraw-Hill Interamericana, 2007.
Vander¿s Human Physiology -
WIDMAIER, E.P.; RAFF, H. and STRANG K.T. ,
McGraw Hill, 2011.
Bioimage Data Analysis -
MIURA K. ,
Wiley-VCH, 2016.